Journal: bioRxiv

Article Title: A multimodal characterization of the human uncinate fasciculus

doi: 10.64898/2026.03.05.708309

Figure Lengend Snippet: Representative micrographs of A) NogoA immunoreactive OLs and B) PDGFRα immunoreactive OPCs. Scale bar = 10 µm. Images were published in Dr. John Kim’s thesis . Bar plots showing groups means ± standard error of the mean for C) OL soma volume and D) OPC soma volume. Bar plots showing groups means ± standard error of the mean for E) OL density and F) OPC density. Scatter plots with trend line showing the relationships between age and G) OL density and H) OPC density. Magenta trend line denotes positive model coefficient for age while blue trend line denotes negative model coefficient for age. Only the age and OL density relationship was statistically significant (p = 0.010).

Article Snippet: After a series of 3 washes in PBS, the tissues were incubated in biotinylated goat anti-rabbit secondary antibody for NogoA (Vector Laboratories BA-1000) and biotinylated horse anti-goat secondary antibody for PDGFRα (Vector Laboratories BA-9500) diluted in blocking buffer for 2 hours at room temperature.

Techniques:

Journal: bioRxiv

Article Title: A multimodal characterization of the human uncinate fasciculus

doi: 10.64898/2026.03.05.708309

Figure Lengend Snippet: Diagram created in https://BioRender.com . Across all the UF experimental modalities, including those reported in our companion paper , throughout the age span we observe: 1) an increase in NogoA+ OL density; 2) changes in gene expression across glial (primarily OL-lineage) cell types that point to transcriptome changes in a wide variety of biological processes including ion channel transport, oxidative phosphorylation, ubiquitination, unfolded protein stress response, immune response, among others; 3) lipid changes with an overall pattern of an increase in monounsaturated fatty acids (MUFA) such as C16:1n-7 and C18:1n-7, and decreases in long chain omega-6 fatty acids such as C22:4n-6; 4) trends of decreasing myelin-constituent proteins and the genes that encode them; and 5) ultrastructural changes of UF myelin in which smaller diameter axons predominate and g-ratio decreases (myelin thickness shows decreasing relationship with age as well, but does not reach statistical significance). Altogether, this pattern of cellular, molecular, and ultrastructural findings suggests profound age-related alterations in the human UF.

Article Snippet: After a series of 3 washes in PBS, the tissues were incubated in biotinylated goat anti-rabbit secondary antibody for NogoA (Vector Laboratories BA-1000) and biotinylated horse anti-goat secondary antibody for PDGFRα (Vector Laboratories BA-9500) diluted in blocking buffer for 2 hours at room temperature.

Techniques: Gene Expression, Phospho-proteomics, Ubiquitin Proteomics

Journal: bioRxiv

Article Title: TBC1D24 regulates mitochondria and endoplasmic reticulum-mitochondria contact sites

doi: 10.1101/2024.09.19.613961

Figure Lengend Snippet: (A-B) Representative images showing the localisation of Flag-TBC1D24 (Flag, Green) in transfected primary fibroblasts, along with mitochondria (TOM20, Magenta)(A) or ER tubules (RTN4, Magenta)(B). (C-D) TBC1D24 mutations affect the structure of ER tubules. (C) Representative images ER tubules (RTN4, Red) and nuclei (Dapi, Blue) in control and patient fibroblasts. Scale bars 10 µm (D) Quantification of ER tubule phenotypes from images in (C). Cells with ER tubules similar to the patient fibroblast shown in (C) were considered as altered. Each point represents an individual experiment. Bars show the average ± SD. *** P<0.001. One-way ANOVA. (E-F) TBC1D24 mutations affect the structure of ER sheets. (E) Representative images ER tubules (CLIMP63, Red) and nuclei (Dapi, Blue) in control and patient fibroblasts. Scale bars 10 µm (F) Quantification of ER sheets phenotypes from images in (E). Cells with ER tubules similar to the patient fibroblasts shown in (E) were considered as altered. Each point represents an individual experiment. Bars show the average ± SD. * p<0.05, *** P<0.001. One-way ANOVA.

Article Snippet: The cells were then incubated for 1 hour at room temperature in blocking buffer with the following primary antibodies: TOM20 (Rb, Abcam, ab186735, 1:250), CLIM63 (Mo, ENZO, ENZ-ABS669, 1: 200), RTN4 (Rb, Bio-Rad, AHP1799, 1:200), FLAG M2 (Mo, Sigma-Aldrich, F1804, 1:200).

Techniques: Transfection, Control